11 November 2005 Development of an electrochemical biosensor without a sandwich assay
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Proceedings Volume 6007, Smart Medical and Biomedical Sensor Technology III; 600706 (2005) https://doi.org/10.1117/12.630748
Event: Optics East 2005, 2005, Boston, MA, United States
Abstract
The combination of electrochemistry with microfluidic sample processing is a viable option to reduce the size, logistics load and power consumption of biosensors. Modern microfluidics technology makes it possible to perform sample clean-up, PCR, sample concentration and transduction on the same disposable chip. This presentation will discuss two novel electrochemical techniques which do not require a sandwich assay and can be employed on a disposable microfluidic chip, reducing logistics load and microfluidic complexity. Transduction is achieved via an electrochemical DNA hybridization sensor similar to a molecular beacon removing the need for a sandwich assay also referred to as E-DNA. The sensor is designed where a DNA stem-loop structure is immobilized on a gold electrode with a redox label held close to the surface. Upon hybridization the stem-loop opens and the label pulls away from the surface so that current cannot flow to the electrode under positive bias. This paper will primarily discuss experiments trying to understand the hybridization event and effect of surface morphology on electrochemical signal transduction.
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James J. Sumner, Kevin W. Plaxco, Carl D. Meinhart, Hyongsok Soh, "Development of an electrochemical biosensor without a sandwich assay", Proc. SPIE 6007, Smart Medical and Biomedical Sensor Technology III, 600706 (11 November 2005); doi: 10.1117/12.630748; https://doi.org/10.1117/12.630748
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