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11 November 2005 Automated spectral acquisition: a smart biomedical sensor technology
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Proceedings Volume 6007, Smart Medical and Biomedical Sensor Technology III; 60070H (2005)
Event: Optics East 2005, 2005, Boston, MA, United States
Raman spectroscopy is a powerful technique for rapid, non-invasive and reagentless analysis of materials, including biological cells. In many samples of biological origin, laser illumination leads to luminescence in addition to Raman scattering. This luminescence will often dissipate after prolonged laser exposure. A common practice is to allow a sample to "photobleach" prior to acquisition of a high quality Raman spectrum. In an effort to automate data acquisition on such samples we are investigating an automated means of quantifying photobleaching and acquiring Raman spectra after photobleaching. We present results of a comparison between an automated approach to acquiring a spectrum on a sample with dynamic luminescence and a manual approach taken by a trained spectroscopist. This component of smart biomedical sensor technology will help allow high quality spectral data to be acquired reproducibly to potentially aid non-spectroscopists with application of Raman spectroscopic approaches.
© (2005) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Lindy McClelland, Shona Stewart, John Maier, Matthew Nelson, and Patrick Treado "Automated spectral acquisition: a smart biomedical sensor technology", Proc. SPIE 6007, Smart Medical and Biomedical Sensor Technology III, 60070H (11 November 2005);

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