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17 November 2005 Carbon nanotube platform for fluorescence based biosensor systems
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Proceedings Volume 6008, Nanosensing: Materials and Devices II; 600804 (2005)
Event: Optics East 2005, 2005, Boston, MA, United States
Single walled carbon nanotube (SWNT), a potential component of nano-biosensor systems, has been successfully demonstrated as a nanoscale platform of biochips which efficiently accommodate biomolecules, such as proteins and peptides. Highly pure SWNTs directly grown in high yield on a SiO2/Si substrate (SWNT film) by chemical vapor deposition (CVD) process have shown that biomolecules are immobilized as their shapes are well retained. Furthermore, SWNT film substrates have embodied a BSA-free chip system by utilizing non-covalent functionalization of SWNT surfaces with 1,1'-carbonyldiimidazole(CDI)-Tween20. Using SWNT films, high specific/nonspecific discrimination ratio has been obtained from biotin-Streptavdin and Protein A (SpA)-Immunoglobulin G (IgG) pairs as well as from small peptide-protein interactions of 3×FLAG-antiFLAG pair. Systematic studies comparing with the state-of-art protein chip have confirmed that the performance of SWNT protein chip is superior in terms of high specific binding efficiency as well as low background signal due to the efficient prevention of nonspecific bindings.
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Hee Cheul Choi and Hye Ryung Byon "Carbon nanotube platform for fluorescence based biosensor systems", Proc. SPIE 6008, Nanosensing: Materials and Devices II, 600804 (17 November 2005);

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