The electrochemiluminescence (ECL) of Ru(bpy)32+ (bpy = 2,2'-bipyridine and its derivatives) complexes has attracted interest from chem- and bio-analytical researchers. Particularly, by labeling bio-molecules with Ru(bpy)32+ derivatives, highly competitive ECL immunoassay and DNA probing have been employed in clinical and research laboratories and are now becoming standard methods. In the well-established commercial systems designed for bench-top applications, paramagnetic microbeads are used for capturing the analytes and separating the excess of labeled biomolecules from the flow cell. The large surface area of the beads provides a high capacity and efficiency for analyte capturing. However, the use of microbeads prevents the instrument from being miniaturized. Furthermore, only a tiny portion of species is enabled to generate luminescence because of the inaccessibility of the majority of the labels to the electrode surface. We propose to develop a handheld device with disposable chips based on the ECL signal modality. Central to this instrumentation is the fabrication of a nanostructured electrode with spatially selective bioimmobilization. The electrode surface is structured to reach the maximum capturing ability and, at the same time, maintain the effective electroactive region and the accessibility for the ruthenium label to be excited electrochemically. In this presentation, we present a manufacturable approach to the fabrication of such disposable nanostructured electrodes for ECL-based handheld devices.