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21 November 2005 Multimodal optical imaging of mouse Ischemic cortex
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Proceedings Volume 6009, Optical Methods in Drug Discovery and Development; 60090X (2005)
Event: Optics East 2005, 2005, Boston, MA, United States
Real time investigation of cerebral blood flow (CBF), and oxy/deoxy hemoglobin volume (HbO,HbR) dynamics has been difficult until recently due to limited spatial and temporal resolution of techniques like laser Doppler flowmetry and MRI. This is especially true for studies of disease models in small animals, owing to the fine structure of the cerebral vasculature. The combination of laser speckle flowmetry (LSF) and multi-spectral reflectance imaging (MSRI) yields high resolution spatio-temporal maps of hemodynamic changes in response to events such as sensory stimuli or arterial occlusion. Ischemia was induced by distal occlusion of the medial cerebral artery (dMCAO). Rapid changes in CBF, HbO, and HbR during the acute phase were captured with high temporal and spatial resolution through the intact skull. Hemodynamic changes that were correlated with vasoconstrictive events, peri-infarct spreading depressions (PISD), were observed. These experiments demonstrate the utility of LSF and Multi-spectral reflectance imaging (MSRI) in mouse disease models.
© (2005) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Phillip B. Jones, Hwa Kyuong Shin, Andrew K. Dunn, Bradley T. Hyman, David A. Boas, Michael A. Moskowitz, and Cenk Ayata "Multimodal optical imaging of mouse Ischemic cortex", Proc. SPIE 6009, Optical Methods in Drug Discovery and Development, 60090X (21 November 2005);

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