Photobiomodulation on the proliferation and collagen synthesis of normal human skin fibroblast cells

Lei Cheng; Timon Cheng-Yi Liu; Jin-Quan Chi; Yan Li; Hua Jin

doi:10.1117/12.667104

2 February 2006 Photobiomodulation on the proliferation and collagen synthesis of normal human skin fibroblast cells

Lei Cheng, Timon Cheng-Yi Liu, Jin-Quan Chi, Yan Li, Hua Jin

Proceedings Volume 6026, ICO20: Biomedical Optics; 60260A (2006) https://doi.org/10.1117/12.667104
Event: ICO20:Optical Devices and Instruments, 2005, Changchun, China

Abstract

Background and Objective: Cultured normal human skin fibroblast cells (HSFs) were once used to study the mechanism of the effects of low intensity He-Ne laser irradiation (LHNL) on wound healing. The proliferation and collagen synthesis of HFSs were modulated by LHNL in different papers, respectively, and both of them are studied in this paper. Study Design/Materials and Methods: The dosage was studied for the same radiation time 300s. The proliferation and collagen synthesis were measured by 3-[4,5-Dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay and the spectrophotometric method for the determination of hydroxyproline, respectively. Results: The dose zones were called dose 1, dose 2 and dose 3 from low dose on so that HSF proliferation was inhibited in dose 1 (16, 24 mJ/cm²), and promoted in dose 2 (298, 503, 597mJ/cm²), and the collagen synthesis was inhibited in dose 2 (401, 526 mJ/cm²), and promoted in dose 3 (714, 926, 1539, 1727mJ/cm²), which supports our biological model of photobiomodulation. It was found there is the linear relationship of the effect with dose with dose in each dose zone. Conclusions: The photobiomodulation on the proliferation and collagen synthesis of HSFs might be linearly dose-dependent in limited dosage with radiation time kept constant, which provides a foundation to discuss photobiomodulation on wound healing.

Citation Download Citation

Lei Cheng, Timon Cheng-Yi Liu, Jin-Quan Chi, Yan Li, and Hua Jin "Photobiomodulation on the proliferation and collagen synthesis of normal human skin fibroblast cells", Proc. SPIE 6026, ICO20: Biomedical Optics, 60260A (2 February 2006); https://doi.org/10.1117/12.667104

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