19 January 2006 Structural properties of liposomes from digital holographic microscopy
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Abstract
We have constructed liposomes from L alpha Phosphatidylcholine (PC) lipids, which are biomimetic lipids similar to those present in the membranes of mammalian cells. We propose an advance in the use of liposomes, such as for drug delivery, to incorporate into the liposomal membranes transport proteins that have been extracted from the lipid membranes of mammalian cells. In this paper, we describe the usage of a novel optical microscope to characterize the nanomechanical properties of these liposomes. We have applied the technique of digital holographic microscopy, using an instrument recently developed at the University of Münster, Germany. This system enabled us to measure quantitatively the structural changes in liposomes. We have investigated the deformations of these biomimetic lipids comprising these liposomes by applying osmotic stresses, in order to gain insight into the membrane environment prior to incorporation of cloned membrane transport proteins. This control of the nanomechanical properties is important in the stresses transmitted to mechanosensitive ion channels that we have incorporated into the liposomal membranes. These liposomes provide transporting vesicles that respond to mechanical stresses, such as those that occur during implantation.
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Isabelle L. Di Maio, Isabelle L. Di Maio, Daniel Carl, Daniel Carl, Patrik Langehanenberg, Patrik Langehanenberg, Stella M. Valenzuela, Stella M. Valenzuela, Andrew R. Battle, Andrew R. Battle, Sabah Al Khazaaly, Sabah Al Khazaaly, Murray Killingsworth, Murray Killingsworth, Bjorn Kemper, Bjorn Kemper, Gert von Bally, Gert von Bally, Donald K. Martin, Donald K. Martin, } "Structural properties of liposomes from digital holographic microscopy", Proc. SPIE 6036, BioMEMS and Nanotechnology II, 60361R (19 January 2006); doi: 10.1117/12.638823; https://doi.org/10.1117/12.638823
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