The collagen structure of equine articular cartilage characterized using polarization-sensitive optical coherence tomography and non-linear microscopy

Jessica C. Mansfield; Nadya Ugryumova; Karen M. Knapp; Stephen J. Matcher

doi:10.1117/12.710873

27 October 2006 The collagen structure of equine articular cartilage characterized using polarization-sensitive optical coherence tomography and non-linear microscopy

Jessica C. Mansfield, Nadya Ugryumova, Karen M. Knapp, Stephen J. Matcher

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Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 604715 (2006) https://doi.org/10.1117/12.710873
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

Equine articular cartilage has been imaged using both polarization-sensitive optical coherence tomography (PS-OCT) and non-linear microscopy. PS-OCT has been used to spatially map the birefringence in the cartilage and we have found that in the vicinity of the lesion the images display a characteristic disruption in the regular birefringence bands shown by normal cartilage. We also note that significant (e.g. x2) variations in the apparent birefringence of samples taken from young (18 month) animals that otherwise appear visually homogeneous are found over spatial scales of a few millimeters. We have also imaged the cartilage using non-linear microscopy and compare the scans taken with second harmonic generation (SHG) light and the two photon fluorescence (TPF) light. SHG images collected using 800 nm excitation reveals the spatial distribution of collagen fibers, whilst TPF images clearly shows the distribution of intracellular and pericellular fluorophores.

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Jessica C. Mansfield, Nadya Ugryumova, Karen M. Knapp, and Stephen J. Matcher "The collagen structure of equine articular cartilage characterized using polarization-sensitive optical coherence tomography and non-linear microscopy", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 604715 (27 October 2006); https://doi.org/10.1117/12.710873

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KEYWORDS

Cartilage

Collagen

Second-harmonic generation

Microscopy

Birefringence

Tissues

Optical fibers

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