27 October 2006 Multifocal two-photon excitation fluorescence sampling imaging combining lifetime and spectrum resolutions
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Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60472T (2006) https://doi.org/10.1117/12.710079
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China
Abstract
Multifocal multiphoton microscopy (MMM) is a more efficient and powerful method for three-dimensional (3-D) fluorescence imaging with reduced acquisition time compared with conventional confocal and two-photon excitation fluorescence microscopy. We present a novel multifocal two-photon excitation fluorescence sampling imaging technique that is based on a specially designed streak camera and combines fluorescence lifetime and spectrum resolutions. A proof-of-principle experiment is performed on a standard fluorescent dye solution (Rhodamine 6G in ethanol), Time- and spectrum-resolved sampled fluorescence image of Rhodamine 6G is obtained in a snapshot. The reconstructed two-dimensional (2-D) fluorescence image of a prepared plant slide is also obtained by moving the sample laterally. The capability of this system capable of performing simultaneous 2-D measurements of temporal and spectral information has many potential applications, e.g., multi-well imaging and spectrally resolved multifocal multiphoton fluorescence lifetime imaging etc.
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Lixin Liu, Lixin Liu, Ziyang Lin, Ziyang Lin, Junle Qu, Junle Qu, Danni Chen, Danni Chen, Gaixia Xu, Gaixia Xu, Baoping Guo, Baoping Guo, Hanben Niu, Hanben Niu, } "Multifocal two-photon excitation fluorescence sampling imaging combining lifetime and spectrum resolutions", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472T (27 October 2006); doi: 10.1117/12.710079; https://doi.org/10.1117/12.710079
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