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27 October 2006Autofluorescence spectroscopic characteristics of nasopharyngeal carcinoma and normal tissue
Light-induced autofluorescence spectra of nasopharyngeal carcinoma and normal tissue in vitro were compared to that of
known endogenous fluorophores to explore the possible causes of tissue autofluorescence and to further determine the
optimal excitation wavelengths for optical biopsy in vivo. Nasopharyngeal carcinoma and normal tissues were obtained
from the suspected patients during pathological biopsy. A FL/FS92O combined TCSPC spectrofluonmeter and a lifetime
spectrometer system was used for autofluorescence spectra measurement. Fluorescence excitation wavelengths varying
from 260 to 480 nm were used to induce tissue autofluorescence, and the corresponding fluorescence emission spectra
were recorded from a range starting 20 nm above the excitation wavelength and extending to 700 nm. The
autofluorescence excitation-emission pairs of nasopharyngeal carcinoma and nonnal tissues occur at 300-330, 340-460
and 450-520 nm, and the optimal diagnostic excitation wavelengths for detection of nasopharyngeal carcinoma were 340
and 450 nm. The results abtained in this study could be treated as a reference for the development of optical biopsy
system for nasopharyngeal carcinoma.
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Buhong Li, Zhenxi Zhang, Fuwen Yang, Lisheng Lin, Minhui Chen, Shusen Xie, "Autofluorescence spectroscopic characteristics of nasopharyngeal carcinoma and normal tissue," Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472X (27 October 2006); https://doi.org/10.1117/12.710662