Autofluorescence spectroscopic characteristics of nasopharyngeal carcinoma and normal tissue

Buhong Li; Zhenxi Zhang; Fuwen Yang; Lisheng Lin; Minhui Chen; Shusen Xie

doi:10.1117/12.710662

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27 October 2006 Autofluorescence spectroscopic characteristics of nasopharyngeal carcinoma and normal tissue

Buhong Li, Zhenxi Zhang, Fuwen Yang, Lisheng Lin, Minhui Chen, Shusen Xie

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Proceedings Volume 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine; 60472X (2006) https://doi.org/10.1117/12.710662
Event: Fourth International Conference on Photonics and Imaging in Biology and Medicine, 2005, Tianjin, China

Abstract

Light-induced autofluorescence spectra of nasopharyngeal carcinoma and normal tissue in vitro were compared to that of known endogenous fluorophores to explore the possible causes of tissue autofluorescence and to further determine the optimal excitation wavelengths for optical biopsy in vivo. Nasopharyngeal carcinoma and normal tissues were obtained from the suspected patients during pathological biopsy. A FL/FS92O combined TCSPC spectrofluonmeter and a lifetime spectrometer system was used for autofluorescence spectra measurement. Fluorescence excitation wavelengths varying from 260 to 480 nm were used to induce tissue autofluorescence, and the corresponding fluorescence emission spectra were recorded from a range starting 20 nm above the excitation wavelength and extending to 700 nm. The autofluorescence excitation-emission pairs of nasopharyngeal carcinoma and nonnal tissues occur at 300-330, 340-460 and 450-520 nm, and the optimal diagnostic excitation wavelengths for detection of nasopharyngeal carcinoma were 340 and 450 nm. The results abtained in this study could be treated as a reference for the development of optical biopsy system for nasopharyngeal carcinoma.

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Buhong Li, Zhenxi Zhang, Fuwen Yang, Lisheng Lin, Minhui Chen, and Shusen Xie "Autofluorescence spectroscopic characteristics of nasopharyngeal carcinoma and normal tissue", Proc. SPIE 6047, Fourth International Conference on Photonics and Imaging in Biology and Medicine, 60472X (27 October 2006); https://doi.org/10.1117/12.710662

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