1 March 2006 Development of a noninvasive multifunctional measurement method using nanosecond pulsed laser for evaluation of regenerative medicine for articular cartilage
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Abstract
We demonstrated the capability of a photoacoustic method for viscoelastic measurement. The measurement method has already proved to be useful for evaluation of regenerative medicine of articular cartilage. However, characterization of the extracellular matrix as well as determination of the viscoelastic property should be carried out for evaluation of regenerative medicine because the extracellular matrix plays an important role. We therefore developed a method for characterization of the extracellular matrix that can be performed simultaneously with the photoacoustic measurement. Since collagen molecules, which are the major contents of the cartilage extracellular matrix, are well known as endogenous fluorescent molecules, it is possible that fluorescence measurement will enable characterization of the extracellular matrix. Third harmonic Q-switched Nd:YAG laser pulses were used as an excitation light source. The time-resolved fluorescence spectroscopy was obtained by using a photonic multi-channel analyzer. Tissue-engineered cartilages cultured under different conditions for various periods were used as samples. Different culture conditions resulted in different extracellular matrix formations. There were significant differences in the measured fluorescent parameters among the culture conditions of cartilage because chondrocytes produce a specific extracellular matrix depending on its culture condition. The specific extracellular matrix contained a specific type of collagen such as collagen type I or type II, which each have specific fluorescent features. Thus, the fluorescent parameters enabled characterization of synthesis of cartilage-associated extracellular matrix. Therefore, the combination of fluorescence and photoacoustic measurement is expected to become a useful evaluation method in regenerative medicine.
© (2006) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Miya Ishihara, Miya Ishihara, Masato Sato, Masato Sato, Nagatoshi Kaneshiro, Nagatoshi Kaneshiro, Genya Mitani, Genya Mitani, Shunichi Sato, Shunichi Sato, Masayuki Ishihara, Masayuki Ishihara, Joji Mochida, Joji Mochida, Makoto Kikuchi, Makoto Kikuchi, } "Development of a noninvasive multifunctional measurement method using nanosecond pulsed laser for evaluation of regenerative medicine for articular cartilage", Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 60840V (1 March 2006); doi: 10.1117/12.645888; https://doi.org/10.1117/12.645888
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