28 February 2006 Detection of 2-photon oxidation from a NIR laser using confocal microscopy
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Abstract
Recent studies have determined that photochemical oxidation in cultured cells can be detected at peak irradiances as low as 8.5x108 W cm-2 (87-fs pulse). Fluorescent dyes, such as CM-H2DCFDA, enable us to quantify the oxidation response of cells to mode-locked near-infrared (NIR) laser exposure. Using a modified confocal microscope, we characterize the time-dependent 2-photon induced fluorescence generated from a given NIR laser exposure. When cultured cells were then pre-loaded with antioxidants, ascorbic acid or N-acetyl-L-cysteine (NAC), they inhibit nonlinear oxidation with different efficiencies, providing insight regarding mechanisms of damage.
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Kurt J. Schuster, Larry E. Estlack, Benjamin A. Rockwell, and Michael L. Denton "Detection of 2-photon oxidation from a NIR laser using confocal microscopy", Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 60841H (28 February 2006); doi: 10.1117/12.644651; https://doi.org/10.1117/12.644651
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