16 February 2006 Fiber-coupled confocal microscope (FCM) for real time imaging of cellular signals in vivo
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To study cellular morphology and functions in vivo in realtime, we developed a fiber-coupled confocal microscope (FCM), and observed fluorescently-labeled cells inside the body of anesthetized rat. We developed an imaging fiber bundle (IFB), which consisted of an objective lens and a multi-fiber assembly (unit fiber: NA > 0.4, 3 micron in diameter). By combining the IFB with a real-time confocal scanner, we detected intracellular signals of the molecular messenger, and the death signals in the form of fluorescence changes even from cells located deep (> 2 mm) inside the solid organs. The FCM we developed is very promising for detailed studies in both the cell-based researches and clinical researches.
© (2006) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Takashi Sakurai, Takashi Sakurai, Seiji Yamamoto, Seiji Yamamoto, Atsuo Miyakawa, Atsuo Miyakawa, Yoshihiko Wakazono, Yoshihiko Wakazono, Takato O. Yoshida, Takato O. Yoshida, Eiji Kohno, Eiji Kohno, Remi Tsuchiya-Susuki, Remi Tsuchiya-Susuki, Yong Wang, Yong Wang, Hideo Hirukawa, Hideo Hirukawa, Susumu Terakawa, Susumu Terakawa, } "Fiber-coupled confocal microscope (FCM) for real time imaging of cellular signals in vivo", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 608803 (16 February 2006); doi: 10.1117/12.645939; https://doi.org/10.1117/12.645939

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