Light-scattering spectroscopic optical coherence tomography for differentiating cells in 3D cell culture

Chenyang Xu; P. Scott Carney; Wei Tan; Stephen A. Boppart M.D.

doi:10.1117/12.644415

20 February 2006 Light-scattering spectroscopic optical coherence tomography for differentiating cells in 3D cell culture

Chenyang Xu, P. Scott Carney, Wei Tan, Stephen A. Boppart M.D.

Author Affiliations +

Proceedings Volume 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV; 608804 (2006) https://doi.org/10.1117/12.644415
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Combining light scatting spectroscopy (LSS) and spectroscopic optical coherence tomography (LS-SOCT) can provide a wealth of information. A theoretical model for analyzing the scattering in SOCT is proposed based on plane wave decomposition. Based on the model, we discuss the possibility of matching the physical characteristics of scatterers with observed spectroscopic signals. Many complicating factors are considered including the effects of scatterer size, incident light polarization, interference between the fields scattered from closely adjacent scatterers, and the numerical aperture of the OCT system. We found the modulation of the spectrum of the incident light by scattering of a plane wave from a single sphere is a good indicator of particle size and composition. We demonstrate that measuring wavelength-dependent scattering in SOCT can be used for particle sizing and contrast enhancement by differentiating cells in 3D cell culture.

Citation Download Citation

Chenyang Xu, P. Scott Carney, Wei Tan, and Stephen A. Boppart M.D. "Light-scattering spectroscopic optical coherence tomography for differentiating cells in 3D cell culture", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 608804 (20 February 2006); https://doi.org/10.1117/12.644415

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