22 February 2006 Portable real-time fluorescence cytometry of microscale cell culture analog devices
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Abstract
A portable fluorescence cytometric system that provides a modular platform for quantitative real-time image measurements has been used to explore the applicability to investigating cellular events on multiple time scales. For a short time scale, we investigated the real-time dynamics of uptake of daunorubicin, a chemotherapeutic agent, in cultured mouse L-cells in a micro cell culture analog compartment using the fluorescent cytometric system. The green fluorescent protein (GFP) expression to monitor induction of pre-specified genes, which occurs on a much longer time scale, has also been measured. Here GFP fluorescence from a doxycycline inducible promoter in a mouse L-cell line was determined. Additionally, a system based on inexpensive LEDs showed performance comparable to a broadband light source based system and reduced photobleaching compared to microscopic examination.
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Donghyun Kim, Donghyun Kim, Daniel A. Tatosian, Daniel A. Tatosian, Michael L. Shuler, Michael L. Shuler, } "Portable real-time fluorescence cytometry of microscale cell culture analog devices", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 60880X (22 February 2006); doi: 10.1117/12.644521; https://doi.org/10.1117/12.644521
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