21 February 2006 Combined microchannel-type erythrocyte deformability test with optical tweezers
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A combined microchannel-type erythrocyte deformability test with optical tweezers has been developed especially for more sensitive detection of cancerous diseases. To demonstrate the performance and sensitivity of the microchannel-type method, we measured the transit velocity of individual erythrocytes passing through a specific confinement region and calculated the modified elongation index defined by the ratio of the width of the microchannel to the elongated length of the squeezed erythrocytes. To know exactly the effect of optical tweezers on erythrocytes, we investigated several morphologies of optically deformed erythrocytes and measured the shape recovery time of erythrocytes in a static aqueous solution under various powers (~ 24 mW) of 1064-nm laser by a dual-trap optical tweezers. Finally we combined these two methods by considering the key parameters of erythrocyte deformability. The results show that the ambiguity of the overlapped experimental data from microchannel-type erythrocyte deformability test was conspicuously reduced, and that the subtle change (≈ 100-200 ms) in shape recovery time which is one of mechanical properties of erythrocyte membrane surface was remarkably amplified to readily discriminate the difference (≈ 2-3 s) between normal and cancerous blood. This suggests the combined method is more sensitive enough to pinpoint the minor quantitative differences between individual erythrocytes, especially in the field of cancer and cardiovascular diseases.
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Won Gu Lee, Won Gu Lee, Hyunwoo Bang, Hyunwoo Bang, Junha Park, Junha Park, Seok Chung, Seok Chung, Keunchang Cho, Keunchang Cho, Chanil Chung, Chanil Chung, Dong-Chul Han, Dong-Chul Han, Jun Keun Chang, Jun Keun Chang, } "Combined microchannel-type erythrocyte deformability test with optical tweezers", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 608813 (21 February 2006); doi: 10.1117/12.647537; https://doi.org/10.1117/12.647537

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