Optofluidic microscope and its applications in biology

Xin Heng; Kevin W. Reynolds; Xiquan Cui; David Erickson; Demetri Psaltis; Changhuei Yang

doi:10.1117/12.640325

21 February 2006 Optofluidic microscope and its applications in biology

Xin Heng, Kevin W. Reynolds, Xiquan Cui, David Erickson, Demetri Psaltis, Changhuei Yang

Author Affiliations +

Proceedings Volume 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV; 608816 (2006) https://doi.org/10.1117/12.640325
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

In this article, we will demonstrate a novel optical imaging device that can be directly integrated into a microfluidic network, and therefore enables on-chip imaging in a microfluidic system. This micro imaging device, termed optofluidic microscope (OFM) is potentially free of bulk optics and is based on a nanohole array defined in a nontransmissive metallic layer that is patterned onto the floor of the microfluidic channel. The operation of the optofluidic microscope will be explained in details and its performance is examined by using a popular animal model, Caenorhabditis elegans (C. elegans). Images from a large population of nematode worms are efficiently acquired within a short time frame. The quality of the OFM images of C. elegans and the morphological characteristics revealed therein are evaluated. Two groups of early-stage C elegans larvae, wild-type and dpy-24 are successfully separated even though their morphological difference at the larval stage is subtle. The experimental results support our claim that the methodology described therein can be effectively used to develop a powerful tool for fulfilling high-resolution, highthroughput imaging task in microfluidics-based systems.

Citation Download Citation

Xin Heng, Kevin W. Reynolds, Xiquan Cui, David Erickson, Demetri Psaltis, and Changhuei Yang "Optofluidic microscope and its applications in biology", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 608816 (21 February 2006); https://doi.org/10.1117/12.640325

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