Laser-activated bubbles in living cells: new universal cytometric method

Dmitri Lapotko; Ekaterina Lukianova

doi:10.1117/12.658160

21 February 2006 Laser-activated bubbles in living cells: new universal cytometric method

Dmitri Lapotko, Ekaterina Lukianova

Proceedings Volume 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV; 608818 (2006) https://doi.org/10.1117/12.658160
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Laser-activated micro- and nano-bubbles (LAB) in cells may be used as universal and sensitive probes for measuring properties of individual cells. Such bubbles can be detected and imaged by using microscopy and flow cytometry. LABs in living blood and tumor cells were induced by pulsed (532 nm, 10 ns) laser radiation and were detected by a thermal lens optical method. LAB lifetime and maximal diameter varied, correspondingly, within the ranges 0.02-10 ms and 0.44-100 mm. LAB parameters - thresholds and probabilities - were found to depend upon the physiological state of cells. Specificity and sensitivity of LAB cytometry were increased by using light-absorbing nanoparticles conjugated to specific monoclonal antibodies.

Citation Download Citation

Dmitri Lapotko and Ekaterina Lukianova "Laser-activated bubbles in living cells: new universal cytometric method", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 608818 (21 February 2006); https://doi.org/10.1117/12.658160

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KEYWORDS

Pulsed laser operation

Nanoparticles

Blood

Microscopy

Signal detection

Tumors

Laser beam diagnostics

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