21 February 2006 Comparative imaging of the vacuolar reticulum of Saprolegnia ferax
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The genus Saprolegnia in the phylum Oomycota have intracellular structures that are distinct from that of filamentous fungi. The vacuolar reticulum for example in Saprolegnia consists of fine static tubules that taper towards the apex of the hypha and are connected to a large vacuole in the basal region. This paper discusses the contribution of the different microscopic techniques in observing ultrastructural changes resulting from modulating GTP binding proteins associated with vesicle production and placement. TEM, DIC and fluorescent observations complemented each other and provided valuable detailed information as to changes in the vacuolar reticulum and the arrangement of organelles. The use of comparative imaging was essential for obtaining sufficient information to make an accurate assessment of changes resulting from perturbation. Without comparison of multiple imaging techniques the resulting conclusions would have been limited with the added potential of being inaccurate. Imaging properties such as cellular detail, overview and specificity from the various forms of microscopy confirmed and contributed information to the analysis. The argument of whether Saprolegnia use a tubular or a vesicular network system to transfer nascent membrane to the growing tip would have been difficult to determine using only one or two imaging techniques. Comparative analysis has indicated that the vacuolar reticulum, previously considered to be static, is a membrane reservoir that allows for membrane transfer to the apical and subapical regions.
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Osu Lilje, Osu Lilje, Erna Lilje, Erna Lilje, "Comparative imaging of the vacuolar reticulum of Saprolegnia ferax", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 60881F (21 February 2006); doi: 10.1117/12.641350; https://doi.org/10.1117/12.641350

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