22 February 2006 Glutamate-induced intracellular calcium oscillations in astrocytes with confocal microscopy
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Changes in the intracellular Ca2+ concentration ([Ca2+]i) play a crucial role involved in the modulation of signal transduction, development, and plasticity in the CNS. Glial cells can respond to various stimuli with an increase in [Ca2+]i. In this paper, we used confocal microscopy to study calcium transient induced by glutamate in cultured astrocytes. Firstly, 100 μM glutamate induced long-time intracellular calcium oscillations in astrocytes and only a single spike under calcium-free solution. When the concentration of glutamate decreased to 1 μM, only a single spike could be induced. It shows that intracellular calcium oscillations depend on agonist concentration and extracellular Ca2+. Secondly, we investigated amplitude of responses under different stimulation. The amplitude of initial peak induced by 100 μM glutamate decreased in Ca2+-free condition, whereas the duration of kinetics was prolonged. But both the amplitude and area of a single spike induced by 1 μM Glu decreased in Ca2+-free condition. The results show that areaof peak is more accurate than amplitude to display transients of [Ca2+]i. All results above suggest that astrocytes are not passive, they display diverse temporal and spatial increases in [Ca2+]i in response to a variety of stimuli. These [Ca2+]i increases provide a possible means for information coding.
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Yuan Zhang, Yuan Zhang, Wei Zhou, Wei Zhou, Xiuli Liu, Xiuli Liu, Geng Zhu, Geng Zhu, Yuxiang Wu, Yuxiang Wu, Qingming Luo, Qingming Luo, } "Glutamate-induced intracellular calcium oscillations in astrocytes with confocal microscopy", Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 60881I (22 February 2006); doi: 10.1117/12.647055; https://doi.org/10.1117/12.647055

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