23 February 2006 The LSM 5 family: an integrated imaging and spectroscopic platform for the study of cellular dynamic processes
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The elucidation of diffusion processes and molecular interactions and their relation to compartments and structures will be essential to understand cellular functions in detail. Often it is not the average signal that is of interest but the behaviour of single molecules which behave as individuals. Fluorescence based assays have revolutionized the way we can observe molecules at work in their natural cellular settings and they have now also become available for single molecule studies. These technologies comprise Fluorescence Fluctuation Analysis (FFA) including Fluorescence Correlation Spectroscopy (FCS), Fluorescence Redistribution After Photobleaching (FRAP), Foerster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging (FLIM). Especially for dual colour experiments and when dealing with delicate samples the employment of multiphoton microscopy using the aforementioned technologies can be of great benefit. Ideal instruments to study single molecules would therefore need to accommodate equipment that allow for fast time resolution, adequate detectors and lasers as well as integrated work flows. In this contribution we discuss the newest developments in commercial instrumentation and software at Carl Zeiss towards highly sensitive imaging in combination with spectroscopic analysis.
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Klaus Weisshart, Klaus Weisshart, } "The LSM 5 family: an integrated imaging and spectroscopic platform for the study of cellular dynamic processes", Proc. SPIE 6089, Multiphoton Microscopy in the Biomedical Sciences VI, 608909 (23 February 2006); doi: 10.1117/12.646299; https://doi.org/10.1117/12.646299

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