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23 February 2006 Simultaneous excitation of multiple fluororophores with a compact femtosecond laser
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We report on a flexible multiphoton imaging system, suitable for simultaneous and efficient excitation of red (DsRed), yellow (YFP), green (GFP) and blue (DAPI) fluorophores. We used a simple, compact laser system, consisting of a 1 μm high energy diode-pumped oscillator and a tunable wavelength extension using a photonics crystal fibre. The combination of a near IR excitation wavelength, high energy per pulse for efficient three photon excitation and spectral extension for GFP excitation allows for high flexibility.We present experimental results of simultaneous and efficient imaging fluorophores couples from the UV to the red (DAPI-RFP, GFP-RFP).
© (2006) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Philippe Legros, Daniel Choquet, Stéphane Gueguen, Eric Mottay, and Nelly Deguil "Simultaneous excitation of multiple fluororophores with a compact femtosecond laser", Proc. SPIE 6089, Multiphoton Microscopy in the Biomedical Sciences VI, 60890U (23 February 2006);

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