Accurate cell counts in live mouse embryos using optical quadrature and differential interference contrast microscopy

William C. Warger II; Judith A. Newmark; Bing Zhao; Carol M. Warner; Charles A. DiMarzio

doi:10.1117/12.644922

23 February 2006 Accurate cell counts in live mouse embryos using optical quadrature and differential interference contrast microscopy

William C. Warger II, Judith A. Newmark, Bing Zhao, Carol M. Warner, Charles A. DiMarzio

Author Affiliations +

Proceedings Volume 6090, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII; 609009 (2006) https://doi.org/10.1117/12.644922
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Present imaging techniques used in in vitro fertilization (IVF) clinics are unable to produce accurate cell counts in developing embryos past the eight-cell stage. We have developed a method that has produced accurate cell counts in live mouse embryos ranging from 13-25 cells by combining Differential Interference Contrast (DIC) and Optical Quadrature Microscopy. Optical Quadrature Microscopy is an interferometric imaging modality that measures the amplitude and phase of the signal beam that travels through the embryo. The phase is transformed into an image of optical path length difference, which is used to determine the maximum optical path length deviation of a single cell. DIC microscopy gives distinct cell boundaries for cells within the focal plane when other cells do not lie in the path to the objective. Fitting an ellipse to the boundary of a single cell in the DIC image and combining it with the maximum optical path length deviation of a single cell creates an ellipsoidal model cell of optical path length deviation. Subtracting the model cell from the Optical Quadrature image will either show the optical path length deviation of the culture medium or reveal another cell underneath. Once all the boundaries are used in the DIC image, the subtracted Optical Quadrature image is analyzed to determine the cell boundaries of the remaining cells. The final cell count is produced when no more cells can be subtracted. We have produced exact cell counts on 5 samples, which have been validated by Epi-Fluorescence images of Hoechst stained nuclei.

Citation Download Citation

William C. Warger II, Judith A. Newmark, Bing Zhao, Carol M. Warner, and Charles A. DiMarzio "Accurate cell counts in live mouse embryos using optical quadrature and differential interference contrast microscopy", Proc. SPIE 6090, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII, 609009 (23 February 2006); https://doi.org/10.1117/12.644922

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