Dye-Enhanced Reflectance and Fluorescence Confocal Microscopy as an Optical Pathology Tool

Anna N. Yaroslavsky; Elena Salomatina; John Novak; Ivan Amat-Roldan; Ana Castano; Michael Hamblin

doi:10.1117/12.658303

22 February 2006 Dye-enhanced reflectance and fluorescence confocal microscopy as an optical pathology tool

Anna N. Yaroslavsky, Elena Salomatina, John Novak, Ivan Amat-Roldan, Ana Castano, Michael Hamblin

Proceedings Volume 6091, Optical Biopsy VI; 609107 (2006) https://doi.org/10.1117/12.658303
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Early detection and precise excision of neoplasms are imperative requirements for successful cancer treatment. In this study we evaluated the use of dye-enhanced confocal microscopy as an optical pathology tool in the ex vivo trial with fresh thick non-melanoma skin cancer excisions and in vivo trial with B16F10 melanoma cancer in mice. For the experiments the tumors were rapidly stained using aqueous solutions of either toluidine blue or methylene blue and imaged using multimodal confocal microscope. Reflectance images were acquired at the wavelengths of 630nm and 650 nm. Fluorescence was excited at 630 nm and 650 nm. Fluorescence emission was registered in the range between 680 nm and 710 nm. The images were compared to the corresponding en face frozen H&E sections. The results of the study indicate confocal images of stained cancerous tissue closely resemble corresponding H&E sections both in vivo and in vitro. This remarkable similarity enables interpretation of confocal images in a manner similar to that of histopathology. The developed technique may provide an efficient real-time optical tool for detecting skin pathology.

Citation Download Citation

Anna N. Yaroslavsky, Elena Salomatina, John Novak, Ivan Amat-Roldan, Ana Castano, and Michael Hamblin "Dye-enhanced reflectance and fluorescence confocal microscopy as an optical pathology tool", Proc. SPIE 6091, Optical Biopsy VI, 609107 (22 February 2006); https://doi.org/10.1117/12.658303

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