21 February 2006 Light scattering properties of kidney epithelial cells and nuclei
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Proceedings Volume 6091, Optical Biopsy VI; 609108 (2006) https://doi.org/10.1117/12.644863
Event: SPIE BiOS, 2006, San Jose, California, United States
Enlargement of mammalian cells nuclei due to the cancerous inflammation can be detected early through noninvasive optical techniques. We report on the results of cellular experiments, aimed towards the development of a fiber optic endoscopic probe used for precancerous detection of Barrett's esophagus. We previously presented white light scattering results from tissue phantoms (polystyrene polybead microspheres). In this paper, we discuss light scattering properties of epithelial MDCK (Madine-Darby Canine Kidney) cells and cell nuclei suspensions. A bifurcated optical fiber is used for experimental illumination and signal detection. The resulting scattering spectra from the cells do not exhibit the predicted Mie theory oscillatory behavior inherent to ideally spherical scatterers, such as polystyrene microspheres. However, we are able to demonstrate that the Fourier transform spectra of the cell suspensions are well correlated with the Fourier transform spectra of cell nuclei, concluding that the dominate scatterer in the backscattering region is the nucleus. This correlation experimentally illustrates that in the backscattering region, the cell nuclei are the main scatterer in the cells of the incident light.
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Elina A. Vitol, Elina A. Vitol, Timothy P. Kurzweg, Timothy P. Kurzweg, Bahram Nabet, Bahram Nabet, "Light scattering properties of kidney epithelial cells and nuclei", Proc. SPIE 6091, Optical Biopsy VI, 609108 (21 February 2006); doi: 10.1117/12.644863; https://doi.org/10.1117/12.644863

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