Real-time imaging of tissue microstructures using intrinsic optical signatures

Bevin Lin; Chad A. Lieber; Jason T. Fitzgerald; Andromachi P. Michalopoulou; Rajesh N. Raman; Christopher D. Pivetti; Christoph Troppmann; Dennis L. Matthews; Stavros G. Demos

doi:10.1117/12.644954

22 February 2006 Real-time imaging of tissue microstructures using intrinsic optical signatures

Bevin Lin, Chad A. Lieber, Jason T. Fitzgerald, Andromachi P. Michalopoulou, Rajesh N. Raman, Christopher D. Pivetti, Christoph Troppmann, Dennis L. Matthews, Stavros G. Demos

Author Affiliations +

Proceedings Volume 6091, Optical Biopsy VI; 60910G (2006) https://doi.org/10.1117/12.644954
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We explore imaging of tissue microstructures using autofluorescence and light scattering methods implemented through a hyperspectral microscope design. This system utilizes long working distance objectives that enable off-axis illumination of tissue thereby allowing for excitation at any optical wavelength without requiring change of optical elements within the microscope. Spectral and polarization elements are easily and rapidly incorporated to take advantage of spectral variations of spectroscopic optical signatures for enhanced contrast. The collection efficiency has been maximized such that image acquisition may be acquired within very short exposure times, a key feature for transferring this technology to a clinical setting. Preliminary studies using human and animal tissues demonstrate the feasibility of this approach for real-time imaging of intact tissues as they would appear in the operating room.

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Bevin Lin, Chad A. Lieber, Jason T. Fitzgerald, Andromachi P. Michalopoulou, Rajesh N. Raman, Christopher D. Pivetti, Christoph Troppmann, Dennis L. Matthews, and Stavros G. Demos "Real-time imaging of tissue microstructures using intrinsic optical signatures", Proc. SPIE 6091, Optical Biopsy VI, 60910G (22 February 2006); https://doi.org/10.1117/12.644954

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