Absorption spectroscopy of individual nano-objects and improved readout of DNA microarrays using photothermal detection

Stephane Berciaud; Laurent Cognet; Gerhard A. Blab; David Lasne; José Remacle; Philippe Tamarat; Brahim Lounis

doi:10.1117/12.647808

27 February 2006 Absorption spectroscopy of individual nano-objects and improved readout of DNA microarrays using photothermal detection

Stephane Berciaud, Laurent Cognet, Gerhard A. Blab, David Lasne, José Remacle, Philippe Tamarat, Brahim Lounis

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Proceedings Volume 6092, Ultrasensitive and Single-Molecule Detection Technologies; 60920A (2006) https://doi.org/10.1117/12.647808
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We developed a photothermal method based on scattering around a nano-absorber that allows for the unprecedented detection of individual nano-objects such as gold nanoparticles with diameter down to 1.4 nm as well as CdSe nanocrystals. This method relies on the absorptive properties of the nano-object and does not suffer from the drawbacks of luminescence-based methods. We present here two different applications of this versatile detection method. First, we performed absorption spectroscopy of individual gold nanoparticles as small as 5nm and CdSe nanocrystals in the multiexcitonic regime. Second, we show the applicability of our method for new types of gold nanoparticles based DNA microarrays. In addition to the intrinsic signal stability due to the use of gold labelling, our technique does not require silver staining enhancement and permits to push the signal dynamics of such microarrays from the single nanoparticle detection to almost the full surface coverage.

Citation Download Citation

Stephane Berciaud, Laurent Cognet, Gerhard A. Blab, David Lasne, José Remacle, Philippe Tamarat, and Brahim Lounis "Absorption spectroscopy of individual nano-objects and improved readout of DNA microarrays using photothermal detection", Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920A (27 February 2006); https://doi.org/10.1117/12.647808

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KEYWORDS

Gold

Nanoparticles

Nanocrystals

Absorption

Absorption spectroscopy

Luminescence

Silver

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