28 February 2006 Probing dynamics of individual bio molecules by single molecule spectroscopy
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The dynamical behavior of individual enzymes is studied by confocal microscopy. This technique makes it possible to gain insights into the detailed spectrum of molecular conformational changes and activities. We report the direct observation of a single CalB lipase-catalyzed reaction for extended periods of time (hours). A model in which a broad spectrum of conformations is involved is has to be invoked in order to explain the stretched exponential behavior of the off-times histograms.
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W. Verheijen, H. Uji-i, C. Flors, J. Hotta, J. Hofkens, "Probing dynamics of individual bio molecules by single molecule spectroscopy", Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920I (28 February 2006); doi: 10.1117/12.668270; https://doi.org/10.1117/12.668270

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