27 February 2006 Silicon photomultipliers for improved biomolecule detection
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Proceedings Volume 6092, Ultrasensitive and Single-Molecule Detection Technologies; 60920L (2006); doi: 10.1117/12.644466
Event: SPIE BiOS, 2006, San Jose, California, United States
Abstract
There is a need for low cost, miniature, integrated optical systems for bioassay monitoring to meet the growing in vitro and point-of-care diagnostics markets. To this end, we are investigating the use of silicon photomultipliers (SiPM) as device upon which to base our technology development. SiPMs have been used successfully in many high-energy physics applications, but their application as a fully integrated biological detection platform has not been shown. In this paper we will present a new detection platform for the measurement of fluorescent biomolecules at much lower concentrations than commercially available systems. Our results show approaches that demonstrate the use of SiPM for the detection of fluorescent proteins and fluorescent-labelled DNA sequences. The SiPM and sample platforms are integrated so that the minimum distance separating the detector from the sample is realised. In addition, direct immobilisation of the DNA sequences onto the SiPM surface is achieved. This combined approach shows improved sensitivities for both the fluorescent proteins and fluorescent-labelled DNA. We are presenting results that show the use of SiPM as a successful technology for the measurement of fluorescent biomolecules at improved lower concentrations.
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F. Lin, C. Jackson, M. Mac Sweeney, M. Manning, M. M. Sheehan, A. Mathewson, "Silicon photomultipliers for improved biomolecule detection", Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920L (27 February 2006); doi: 10.1117/12.644466; https://doi.org/10.1117/12.644466
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KEYWORDS
Light emitting diodes

Luminescence

Proteins

Silicon photomultipliers

Sensors

Signal detection

Green fluorescent protein

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