A space- and time-resolved single photon counting detector for fluorescence microscopy and spectroscopy

X. Michalet; O.H.W. Siegmund; J. V. Vallerga; P. Jelinsky; J. E. Millaud; S. Weiss

doi:10.1117/12.646482

27 February 2006 A space- and time-resolved single photon counting detector for fluorescence microscopy and spectroscopy

X. Michalet, O.H.W. Siegmund, J. V. Vallerga, P. Jelinsky, J. E. Millaud, S. Weiss

Author Affiliations +

Proceedings Volume 6092, Ultrasensitive and Single-Molecule Detection Technologies; 60920M (2006) https://doi.org/10.1117/12.646482
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We have recently developed a wide-field photon-counting detector having high-temporal and high-spatial resolutions and capable of high-throughput (the H33D detector). Its design is based on a 25 mm diameter multi-alkali photocathode producing one photo electron per detected photon, which are then multiplied up to 10⁷ times by a 3-microchannel plate stack. The resulting electron cloud is proximity focused on a cross delay line anode, which allows determining the incident photon position with high accuracy. The imaging and fluorescence lifetime measurement performances of the H33D detector installed on a standard epifluorescence microscope will be presented. We compare them to those of standard single-molecule detectors such as single-photon avalanche photodiode (SPAD) or electron-multiplying camera using model samples (fluorescent beads, quantum dots and live cells). Finally, we discuss the design and applications of future generation of H33D detectors for single-molecule imaging and high-throughput study of biomolecular interactions.

Citation Download Citation

X. Michalet, O.H.W. Siegmund, J. V. Vallerga, P. Jelinsky, J. E. Millaud, and S. Weiss "A space- and time-resolved single photon counting detector for fluorescence microscopy and spectroscopy", Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920M (27 February 2006); https://doi.org/10.1117/12.646482

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