Control the Number of Biomolecules Tethering on Functionalized AFM Tips

Z. D. Xiao; X. X. Xiao; Z. H. Lu; C. M. Yam; C. Z. Cai

doi:10.1117/12.657867

27 February 2006 Control the number of biomolecules tethering on functionalized AFM tips

Z. D. Xiao, X. X. Xiao, Z. H. Lu, C. M. Yam, C. Z. Cai

Proceedings Volume 6092, Ultrasensitive and Single-Molecule Detection Technologies; 60920Q (2006) https://doi.org/10.1117/12.657867
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We have developed a practical method to control the number of attaching biomolecules to an AFM tip. Monolayer of OEG that has little interaction with some biomolecules was self-assembled on tips. Electric pulses were used to oxidize a patch of OEG monolayer on tip and generate COOH groups for further linking at the activated area. The surface groups were detected by chemical force titration. The total numbers of molecule bound on the tip was examined by the studies of biotin/streptavidin system.

Citation Download Citation

Z. D. Xiao, X. X. Xiao, Z. H. Lu, C. M. Yam, and C. Z. Cai "Control the number of biomolecules tethering on functionalized AFM tips", Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920Q (27 February 2006); https://doi.org/10.1117/12.657867

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