27 February 2006 Multimodal multiplex Raman spectroscopy optimized for in vivo chemometrics
Author Affiliations +
Abstract
We have designed and constructed a multimodal multiplex Raman spectrometer which uses multi-wavelength excitation to better detect signals in the presence of fluorescence by taking advantage of the shift-variance of the Raman signal with respect to excitation frequency. Coupled with partial-least-squares (PLS) regression, the technique applied to ethanol estimation in a tissue phantom achieves root-mean-squared-cross-validation errors (RMSCVE) of 9.2 mmol/L with a model formed with 2 principal components, compared to a single wavelength data set with equivalent energy where 7 principal components were used to achieve an RMSCVE of 39.1 mmol/L.
© (2006) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
S. T. McCain, M. E. Gehm, Y. Wang, N. P. Pitsianis, D. J. Brady, "Multimodal multiplex Raman spectroscopy optimized for in vivo chemometrics", Proc. SPIE 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry, 60930P (27 February 2006); doi: 10.1117/12.646755; https://doi.org/10.1117/12.646755
PROCEEDINGS
8 PAGES


SHARE
Back to Top