27 February 2006 Raman spectroscopic biochemical mapping of tissues
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Advances in technologies have brought us closer to routine spectroscopic diagnosis of early malignant disease. However, there is still a poor understanding of the carcinogenesis process. For example it is not known whether many cancers follow a logical sequence from dysplasia, to carcinoma in situ, to invasion. Biochemical tissue changes, triggered by genetic mutations, precede morphological and structural changes. These can be probed using Raman or FTIR microspectroscopy and the spectra analysed for biochemical constituents. Local microscopic distribution of various constituents can then be visualised. Raman mapping has been performed on a number of tissues including oesophagus, breast, bladder and prostate. The biochemical constituents have been calculated at each point using basis spectra and least squares analysis. The residual of the least squares fit indicates any unfit spectral components. The biochemical distribution will be compared with the defined histopathological boundaries. The distribution of nucleic acids, glycogen, actin, collagen I, III, IV, lipids and others appear to follow expected patterns.
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Nicholas Stone, Nicholas Stone, Maria Consuelo Hart Prieto, Maria Consuelo Hart Prieto, Catherine Ann Kendall, Catherine Ann Kendall, Geeta Shetty, Geeta Shetty, Hugh Barr, Hugh Barr, "Raman spectroscopic biochemical mapping of tissues", Proc. SPIE 6093, Biomedical Vibrational Spectroscopy III: Advances in Research and Industry, 60930U (27 February 2006); doi: 10.1117/12.644178; https://doi.org/10.1117/12.644178

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