Dendrimer optimization for a glucose-sensitive fluorescent assay

Hope T. Beier; Bennett L. Ibey; Rebecca M. Rounds; Michael V. Pishko; Gerard L. Coté

doi:10.1117/12.645357

27 February 2006 Dendrimer optimization for a glucose-sensitive fluorescent assay

Hope T. Beier, Bennett L. Ibey, Rebecca M. Rounds, Michael V. Pishko, Gerard L. Coté

Proceedings Volume 6094, Optical Diagnostics and Sensing VI; 60940I (2006) https://doi.org/10.1117/12.645357
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

A fluorescent assay based on the competitive binding between glycosylated PAMAM dendrimer and glucose with the sugar-binding lectin Concanavalin A has been developed. This assay, composed of the glycodendrimer and Alexa Fluor 647 labeled Concanavalin A, has shown a large dynamic response to physiological concentrations of glucose. The larger dynamic range is believed to be due to the spheriodal shape of the dendrimer molecule, which eliminates the multiple binding of the same dextran chain to the Concanavalin A tetramer that plagued previous approaches. However, in order to further understand the operation of the assay and optimize the dynamic response, the dendrimer construction must be modified to determine the optimum degree of glycosylation. In this paper, a description of the assay function and the change in fluorescence response with various formulations of glycodendrimers are shown. Theories are also presented as means of understanding the various assay responses with different degrees of dendrimer functionalization.

Citation Download Citation

Hope T. Beier, Bennett L. Ibey, Rebecca M. Rounds, Michael V. Pishko, and Gerard L. Coté "Dendrimer optimization for a glucose-sensitive fluorescent assay", Proc. SPIE 6094, Optical Diagnostics and Sensing VI, 60940I (27 February 2006); https://doi.org/10.1117/12.645357

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available