28 February 2006 Locked nucleic acid molecular beacons for intracellular mRNA monitoring
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Abstract
We have designed, synthesized, and investigated a novel molecular beacon (MB) using locked nucleic acid (LNA) bases for intracellular mRNA monitoring. This new LNA-MB has several useful properties including: very high melting temperature; excellent affinity for complementary sequences; superior single base mismatch discrimination capability; stablity against nuclease digestion; and not binding with single-stranded DNA binding proteins. All of these properties are highly advantageous for a molecular tool for various intracellular studies of biochemical, biological and medical significance.
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Lin Wang, Lin Wang, Chaoyong James Yang, Chaoyong James Yang, Colin D. Medley, Colin D. Medley, Steven A. Benner, Steven A. Benner, Weihong Tan, Weihong Tan, } "Locked nucleic acid molecular beacons for intracellular mRNA monitoring", Proc. SPIE 6095, Nanobiophotonics and Biomedical Applications III, 60950M (28 February 2006); doi: 10.1117/12.644649; https://doi.org/10.1117/12.644649
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