Fluorescence resonance energy transfer using lanthanide-ion doped oxide nanoparticles as donors

D. Casanova; D. Giaume; T. Amirtha; T. Gacoin; J.-P. Boilot; A. Alexandrou D.D.S.

doi:10.1117/12.646479

27 March 2006 Fluorescence resonance energy transfer using lanthanide-ion doped oxide nanoparticles as donors

D. Casanova, D. Giaume, T. Amirtha, T. Gacoin, J.-P. Boilot, A. Alexandrou D.D.S.

Proceedings Volume 6096, Colloidal Quantum Dots for Biomedical Applications; 60960J (2006) https://doi.org/10.1117/12.646479
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We have demonstrated fluorescence resonance energy transfer (FRET) between lanthanide-ion doped oxide nanoparticles acting as donors and organic acceptor molecules (Cy5). Due to the long nanoparticle lifetime and the large Stokes shift between nanoparticle absorption and emission, unambiguous and precise FRET measurements can be performed despite the presence of large free acceptor oncentrations. We determined FRET efficiencies as a function of Cy5 concentration which are in very good agreement with a multiple acceptor-multiple donor calculation.

Citation Download Citation

D. Casanova, D. Giaume, T. Amirtha, T. Gacoin, J.-P. Boilot, and A. Alexandrou D.D.S. "Fluorescence resonance energy transfer using lanthanide-ion doped oxide nanoparticles as donors", Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60960J (27 March 2006); https://doi.org/10.1117/12.646479

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