27 March 2006 Quantum-dot-based nanosensors designed for proteolytic monitoring
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We have previously assembled QD-based fluorescence resonance energy transfer (FRET) sensors specific for the sugar nutrient maltose and the explosive TNT. These sensors utilize several inherent benefits of QDs as FRET donors. In this report, we show that QD-FRET based sensors can also function in the monitoring of proteolytic enzyme activity. We utilize a QD with multiple dye-labeled proteins attached to the surface as a substrate for a prototypical protease. We then demonstrate how this strategy can be extended to detect protease activity by utilizing a dye-labeled peptide attached to the QD as a proteolytic substrate. Self-assembly of the peptide-dye on the QD brings the dye in close proximity to the QD and result in efficient FRET. Addition of a proteolytic enzyme that specifically recognizes and cleaves the peptide alters the FRET signature of the sensor in a concentration-dependent manner. Both qualitative and quantitative data can be derived from these sensors. The potential benefits of this type of QD sensing strategy are discussed.
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Igor L. Medintz, Aaron R. Clapp, Florence M. Brunel, Ellen R. Goldman, Eddie L. Chang, Phillip E. Dawson, Hedi Mattoussi, "Quantum-dot-based nanosensors designed for proteolytic monitoring", Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60960K (27 March 2006); doi: 10.1117/12.646199; https://doi.org/10.1117/12.646199

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