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10 February 2006 Molecular differentiation of Leishmania protozoarium using CdS quantum dots as biolabels
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In this work we applied core-shell CdS/Cd(OH)2 quantum dots (QDs) as fluorescent labels in the Leishmania amazonensis protozoarium. The nanocrystals (8-9 nm) are obtained via colloidal synthesis in aqueous medium, with final pH=7 using sodium polyphosphate as the stabilizing agent. The surface of the particles is passivated with a cadmium hydroxide shell and the particle surface is functionalized with glutaraldehyde. The functionalized and non-functionalized particles were conjugated to Leishmania organisms in the promastigote form. The marked live organisms were visualized using confocal microscopy. The systems exhibit a differentiation of the emission color for the functionalized and non-functionalized particles suggesting different chemical interactions with the promastigote moieties. Two photon emision spectra (λexc=795nm) were obtained for the promastigotes labeled with the functionalized QDs showing a significant spectral change compared to the original QDs suspension. These spectral changes are discussed in terms of the possible energy deactivation processes.
© (2006) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Beate S. Santos, Patrícia M. A. de Farias, Frederico D. de Menezes, Erick L. Mariano, Ricardo de C. Ferreira, Selma Giorgio, Maira C. Bosetto, Diana C. Ayres, Paulo M. Lima, Adriana Fontes, André A. de Thomas, and Carlos L. Cesar "Molecular differentiation of Leishmania protozoarium using CdS quantum dots as biolabels", Proc. SPIE 6097, Optical Molecular Probes for Biomedical Applications, 609706 (10 February 2006);

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