Bio-imaging of DsRed fluorescence in the transgenic rat liver

Yukitomo Arao; Yoji Hakamata D.V.M.; Yuka Igarashi; Yuki Sato M.D.; Takashi Murakami M.D.; Eiji Kobayashi M.D.

doi:10.1117/12.647334

23 February 2006 Bio-imaging of DsRed fluorescence in the transgenic rat liver

Yukitomo Arao, Yoji Hakamata D.V.M., Yuka Igarashi, Yuki Sato M.D., Takashi Murakami M.D., Eiji Kobayashi M.D.

Proceedings Volume 6098, Genetically Engineered Probes for Biomedical Applications; 60980E (2006) https://doi.org/10.1117/12.647334
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

We developed the Alb-DsRed2 transgenic (Tg) rat designed with liver-specific expression of the red fluorescent protein, DsRed2. Herein, we report high expression of DsRed2 in neonate liver of both sexes, although they were sexually dimorphic and exhibited a male-specific pattern in adult rats. In an effort to examine the expression in each animal under development, we employed an in vivo Bio-imaging system to quantitatively estimate hepatic DsRed2 expression levels. The temporal profiles pertaining to DsRed expression were similar in male and female Tg rats until 28 days old. The levels in both sexes decreased gradually following birth, and were not detectable at 21 days. Subsequently, expression in males increased again at 35 days and was maintained at a persistently high level thereafter. On the other hand, expression in females disappeared steadily. Although hepatic DsRed expression levels in gonadectomized Tg rats was not significantly different, DsRed expression in hypophysectomized female Tg rats appeared dramatically 72 hr following operation. Hepatocytes were collected from adult Tg rats and cultured in conditioning medium. DsRed expression in female hepatocytes could be detected 72 hr following culturing. These results suggest that hepatic DsRed expression in female rats is regulated in vivo by the pituitary. This report is shows use of Alb-DsRed2 Tg rats in conjunction with a novel bio-imaging system represents a powerful experimental system.

Citation Download Citation

Yukitomo Arao, Yoji Hakamata D.V.M., Yuka Igarashi, Yuki Sato M.D., Takashi Murakami M.D., and Eiji Kobayashi M.D. "Bio-imaging of DsRed fluorescence in the transgenic rat liver", Proc. SPIE 6098, Genetically Engineered Probes for Biomedical Applications, 60980E (23 February 2006); https://doi.org/10.1117/12.647334

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KEYWORDS

Liver

In vivo imaging

Luminescence

Fluorescent proteins

Microscopes

Biological research

Imaging systems

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