Prolongation of GFP-expressed skin graft after intrathymic injection of GFP positive splenocytes in adult rats

Yoji Hakamata D.V.M.; Yuka Igarashi; Takashi Murakami M.D.; Eiji Kobayashi M.D.

doi:10.1117/12.648014

23 February 2006 Prolongation of GFP-expressed skin graft after intrathymic injection of GFP positive splenocytes in adult rat

Yoji Hakamata D.V.M., Yuka Igarashi, Takashi Murakami M.D., Eiji Kobayashi M.D.

Author Affiliations +

Proceedings Volume 6098, Genetically Engineered Probes for Biomedical Applications; 60980F (2006) https://doi.org/10.1117/12.648014
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

GFP is a fluorescent product of the jellyfish Aequorea victoria and has been used for a variety of biological experiments as a reporter molecule. While GFP possesses advantages for the non-invasive imaging of viable cells, GFP-positive cells are still considered potential xeno-antigens. It is difficult to observe the precise fate of transplanted cells/organs in recipients without immunological control. The aim of this study was to determine whether intrathymic injection of GFP to recipients and the depletion of peripheral lymphocytes could lead to donor-specific unresponsiveness to GFP-expressed cell. LEW rats were administered intraperitoneally with 0.2 ml of anti-rat lymphocyte serum (ALS) 1 day prior to intrathymic injection of donor splenocytes or adeno-GFP vector. Donor cells and vector were non-invasively inoculated into the thymus under high frequency ultrasound imaging using an echo-guide. All animals subsequently received a 7 days GFP-expressed skin graft from the same genetic background GFP LEW transgenic rat. Skin graft survival was greater in rats injected with donor splenocytes (23.6+/-9.1) compared with adeno-GFP (13.0+/-3.7) or untreated control rats (9.5+/-1.0). Intrathymic injection of donor antigen into adult rats can induce donor-specific unresponsiveness. Donor cells can be observed for a long-term in recipients with normal immunity using this strategy.

Citation Download Citation

Yoji Hakamata D.V.M., Yuka Igarashi, Takashi Murakami M.D., and Eiji Kobayashi M.D. "Prolongation of GFP-expressed skin graft after intrathymic injection of GFP positive splenocytes in adult rat", Proc. SPIE 6098, Genetically Engineered Probes for Biomedical Applications, 60980F (23 February 2006); https://doi.org/10.1117/12.648014

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Green fluorescent protein

Skin

Ultrasonography

Imaging systems

Medicine

Tolerancing

Transplantation

Show All Keywords

Keywords/Phrases

Search In:

Publication Years