23 February 2006 Antioxidant assay using genetically engineered bioluminescent Escherichia coli
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A new antioxidant activity assay based on the reactive oxygen species (ROS)-inducible bacterial strain (E. coli DPD2511) is described. The strain harbors the plasmid pKatG::luxCDABE and responds to hydrogen peroxide treatment by increasing light emission at 490 nm. Antioxidant capacity is evaluated through the ability of an agent to inhibit the hydrogen peroxide-induced bioluminescence of E. coli DPD2511. Applicability of the developed assay in detecting levels of antioxidants in various aqueous plant extracts is demonstrated. The assay was validated against 2,2-diphenylpicrylhydrazyl (DPPH) assay, a known antioxidant assay.
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Amelita Bartolome, Amelita Bartolome, Bernadette Macalino, Bernadette Macalino, Ian Lemuel Pastoral, Ian Lemuel Pastoral, Fortunato Sevilla, Fortunato Sevilla, } "Antioxidant assay using genetically engineered bioluminescent Escherichia coli", Proc. SPIE 6098, Genetically Engineered Probes for Biomedical Applications, 60980J (23 February 2006); doi: 10.1117/12.643950; https://doi.org/10.1117/12.643950

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