23 January 2006 Injection and manipulation of silicon microbeads in a customized microfluidic platform
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The first on-chip injection and manipulation of optically encoded, silicon microbeads in a microfluidic platform is reported. Encoded microbeads of different shapes and sizes were fabricated in silicon via standard microfabrication techniques. The optical signature consisted of a series of lithographically defined bar-codes, which can be identified by a laser detection system. In-situ identification of encoded microbeads was possible at microbead velocities ≤ 50 cm per second. The microbeads can also be transported within a channel network in accordance with the encoded optical signature of each bead. The microbead transport is controlled by the laminar flow of a liquid in pressure driven microchannels. Hydrodynamic pulsing facilitated single and multiple injection of microbeads from a reservoir into the laminar fluid stream of a branched microfluidic network. Careful control of the fluid velocity and alteration of the microchannel geometry also enabled manipulation of microbead velocity. The incorporation of five pillars to retain microbeads at a specific location within the microchannel network formed the basis of a reaction chamber for on chip functionalization of microbeads. The principle of hydrodynamic switching was utlized to re-direct the transport of microbeads at a branched microfluidic network. In the final part of this research we verify that this microbead technology is suitable for detection of specific target DNA.
© (2006) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Daniel Hoffmann, Daniel Hoffmann, Des Brennan, Des Brennan, Michael Loughran, Michael Loughran, } "Injection and manipulation of silicon microbeads in a customized microfluidic platform", Proc. SPIE 6112, Microfluidics, BioMEMS, and Medical Microsystems IV, 61120E (23 January 2006); doi: 10.1117/12.660342; https://doi.org/10.1117/12.660342


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