Effect of wavelength and fluence on morphology cellular and genetic integrity of diabetic wounded human skin fibroblasts

H. Abrahamse; D. Hawkins; N. Houreld

doi:10.1117/12.641172

28 February 2006 Effect of wavelength and fluence on morphology cellular and genetic integrity of diabetic wounded human skin fibroblasts

H. Abrahamse, D. Hawkins, N. Houreld

Author Affiliations +

Proceedings Volume 6140, Mechanisms for Low-Light Therapy; 614006 (2006) https://doi.org/10.1117/12.641172
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

An alternative treatment modality for diabetic wound healing includes low level laser therapy (LLLT). Biostimulation of such wounds may be of benefit to patients by reducing healing time. Structural, cellular and genetic events in diabetic wounded human skin fibroblasts (WS1) were evaluated after exposing cells in culture to a Helium-Neon (632.8nm), a Diode laser (830nm) and a Nd:YAG (Neodynium:Yttrium-Allumina-Gallium) laser (1064nm) at either 5J/cm² or 16J/cm². Cells were exposed twice a week and left 24 hours post-irradiation prior to measuring effects. Structural changes were evaluated by assessing colony formation, haptotaxis and chemotaxis. Cellular changes were evaluated using cell viability, (adenosine-triphosphate, ATP production), and proliferation, (alkaline phosphatase, ALP and basic fibroblast growth factor, bFGF expression), while the Comet assay evaluated DNA damage and cytotoxicity was determined assessing membrane permeability for lactate dehydrogenase (LDH). Caspase 3/7 activity was used as an estimate of apoptosis as a result of irradiation. The irradiated diabetic wounded cells showed structural, cellular as well as molecular resilience comparable to that of unwounded normal skin fibroblast cells. With regards to fluence, 5J/cm² elicit positive cellular and structural responses while 16J/cm² increases cellular and genetic damage and cellular morphology is altered. Different wavelengths of LLLT influences the beneficial outcomes of diabetic wounded cells and although all three wavelengths elicit cellular effects, the penetration depth of 830nm plays a significant role in the healing of diabetic wounded human fibroblast cells. Results from this study validate the contribution of LLLT to wound healing and elucidate the biochemical effects at a cellular level while highlighting the role of different dosages and wavelengths in LLLT.

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H. Abrahamse, D. Hawkins, and N. Houreld "Effect of wavelength and fluence on morphology cellular and genetic integrity of diabetic wounded human skin fibroblasts", Proc. SPIE 6140, Mechanisms for Low-Light Therapy, 614006 (28 February 2006); https://doi.org/10.1117/12.641172

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