In vivo micro-CT imaging of the murine lung via a computer controlled intermittent iso-pressure breath hold (IIBH) technique

Eman Namati; Deokiee Chon; Jacqueline Thiesse; Geoffrey McLennan; Jered Sieren; Alan Ross; Eric A. Hoffman

doi:10.1117/12.651885

13 March 2006 In vivo micro-CT imaging of the murine lung via a computer controlled intermittent iso-pressure breath hold (IIBH) technique

Eman Namati, Deokiee Chon, Jacqueline Thiesse, Geoffrey McLennan, Jered Sieren, Alan Ross, Eric A. Hoffman

Author Affiliations +

Proceedings Volume 6143, Medical Imaging 2006: Physiology, Function, and Structure from Medical Images; 614305 (2006) https://doi.org/10.1117/12.651885
Event: Medical Imaging, 2006, San Diego, California, United States

Abstract

Micro-CT, a technique for imaging small objects at high resolution using micro focused x-rays, is becoming widely available for small animal imaging. With the growing number of mouse models of pulmonary pathology, there is great interest in following disease progression and evaluating the alteration in longitudinal studies. Along with the high resolution associated with micro CT comes increased scanning times, and hence minimization of motion artifacts is required. We propose a new technique for imaging mouse lungs in vivo by inducing an intermittent iso-pressure breath hold (IIBH) with a fixed level of positive airway pressure during image acquisition, to decrease motion artifacts and increase image resolution and quality. Mechanical ventilation of the respiratory system for such a setup consists of three phases, 1) tidal breathing (hyperventilated), 2) a breath hold during a fixed level of applied positive airway pressure, 3) periodic deep sighs. Image acquisition is triggered over the stable segment of the IIBH period. Comparison of images acquired from the same mouse lung using three imaging techniques (normal breathing / no gating, normal breathing with gating at End Inspiration (EI) and finally the IIBH technique) demonstrated substantial improvements in resolution and quality when using the IIBH gating. Using IIBH triggering the total image acquisition time increased from 15 minutes to 35 minutes, although total x-ray exposure time and hence animal dosage remains the same. This technique is an important step in providing high quality lung imaging of the mouse in vivo, and will provide a good foundation for future longitudinal studies.

Citation Download Citation

Eman Namati, Deokiee Chon, Jacqueline Thiesse, Geoffrey McLennan, Jered Sieren, Alan Ross, and Eric A. Hoffman "In vivo micro-CT imaging of the murine lung via a computer controlled intermittent iso-pressure breath hold (IIBH) technique", Proc. SPIE 6143, Medical Imaging 2006: Physiology, Function, and Structure from Medical Images, 614305 (13 March 2006); https://doi.org/10.1117/12.651885

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

In vivo imaging

Lung

Image resolution

Image acquisition

Scanners

X-rays

Image quality

Show All Keywords

Keywords/Phrases

Search In:

Publication Years