13 March 2006 Validation of a fiber-based confocal microscope for interventional image-guided procedures: correlation with multispectral optical imaging
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Abstract
The concept of the biopsy is ubiquitous in current medical diagnosis of cancer and other diseases. The standard biopsy consists of removing a sample of tissue for evaluation and diagnosis, primarily to ascertain the presence of cancer cells by (histo)pathological analyses. However, the advent of new optical imaging modalities and targeted or "smart" agents, that have affinity for a select target, suggests the possibility of performing in vivo tissue characterization without the need for sample removal or the wait for histopathologic processing. Here we present work testing and validating a fiber-based confocal fluorescence microscopic imaging system intended for combination with a larger scale imaging modality (i.e. MRI or CT) to be used in image-guided in vivo tissue characterization. Fiber-based confocal fluorescence microscopic imaging experiments were performed (Cellvizio, Mauna Kea Technologies, Paris, France) in vivo in two mouse models including: 1) EGFP-expressing mouse melanoma model and 2) M21 mouse melanoma model. Both models are known to express integrin ανβ3, a cell-surface receptor protein. We also performed an experiment in ex vivo chicken muscle tissue labelled with a fluorescein isothiocyanate-lectin targeted compound. In the mouse models, contrast agents that targeted the integrin were injected and the contrast agent localization in tumor was verified by a whole-body multispectral imager. The fiber-based tool was sensitive enough to detect and image the tissue of interest in all different experiments, and was found appropriate for use in interventional catheter-based procedures.
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Daniel Herzka, Daniel Herzka, Jade Quijano, Jade Quijano, Jianwu Xie, Jianwu Xie, Sascha Krueger, Sascha Krueger, Steffen Weiss, Steffen Weiss, Benjamin Abrat, Benjamin Abrat, Anne Osdoit, Anne Osdoit, Charlotte Cavé, Charlotte Cavé, Christopher Burnett, Christopher Burnett, S. Narasimhan Danthi, S. Narasimhan Danthi, King Li, King Li, } "Validation of a fiber-based confocal microscope for interventional image-guided procedures: correlation with multispectral optical imaging", Proc. SPIE 6143, Medical Imaging 2006: Physiology, Function, and Structure from Medical Images, 61433D (13 March 2006); doi: 10.1117/12.653272; https://doi.org/10.1117/12.653272
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