New method for studying the relationship between morphological parameters and cell viability

Jianwen Xiong; Weiping Dai; Li Chen; Guixiang Liu; Mingsheng Liu; Zhenxi Zhang; Hua Xiao

doi:10.1117/12.676524

23 February 2006 New method for studying the relationship between morphological parameters and cell viability

Jianwen Xiong, Weiping Dai, Li Chen, Guixiang Liu, Mingsheng Liu, Zhenxi Zhang, Hua Xiao

Proceedings Volume 6150, 2nd International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment; 615043 (2006) https://doi.org/10.1117/12.676524
Event: 2nd International Symposium on Advanced Optical Manufacturing and Testing Technologies, 2005, Xian, China

Abstract

Based on the morphological differences between normal, apoptosis and necrosis cell, a new method for detection cell viability is presented in the paper. The Jurkat cells samples were used for studying the relationship between morphological parameters and cell viability in the paper. According to the scatter charts of Jurkat cell roundness, radius ratio and area, the cell areas were mainly distributed from 40 μm² to 80 μm². The percentage of main areas in the two cells samples were analyzed statistically with values of 62.37% and 75.45 % respectively. Due to the mostly normal cell used by the experiment in the exponent growth period, a conclusion that the areas of normal cells were mainly distributed from 40 μm² to 80 μm² is presented. And the areas of apoptosis and necrosis cells are distributed in other range, i.e. less than 40 μm² or more than 80 μm². There are not any chemical medicaments needed to add in the samples by the method based on the videopicture in order to detect the states of cell samples. What it reflected by analyzing morphological parameters of cell are the true states of cell.

Citation Download Citation

Jianwen Xiong, Weiping Dai, Li Chen, Guixiang Liu, Mingsheng Liu, Zhenxi Zhang, and Hua Xiao "New method for studying the relationship between morphological parameters and cell viability", Proc. SPIE 6150, 2nd International Symposium on Advanced Optical Manufacturing and Testing Technologies: Optical Test and Measurement Technology and Equipment, 615043 (23 February 2006); https://doi.org/10.1117/12.676524

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available