21 July 2006 Visualisation of polymer distribution in polyelectrolyte microcapsules by light scattering and multiple fluorescence labelling
Author Affiliations +
Abstract
In this contribution we show application of multispectral confocal laser scanning microscopy for visualisation and characterisation of polyelectrolyte microcapsules. Fluorescent labels of different free charges, specifically bound by electrostatic interactions to the polymer chains in polyelectrolyte microcapsules, were used for questioning the spatial distribution of polymer components of the microcapsules. To separate the overlapping emission spectra of the fluorescent labels spectral imaging and linear unmixing approaches was used.
© (2006) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
J. Podskocova, J. Podskocova, D. Chorvat, D. Chorvat, G. Kollarikova, G. Kollarikova, I. Lacik, I. Lacik, } "Visualisation of polymer distribution in polyelectrolyte microcapsules by light scattering and multiple fluorescence labelling", Proc. SPIE 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII, 61630V (21 July 2006); doi: 10.1117/12.697290; https://doi.org/10.1117/12.697290
PROCEEDINGS
8 PAGES


SHARE
Back to Top