24 July 2006 Cytokine production of the neutrophils and macrophages in time of phagocytosis under influence of infrared low-level laser irradiation
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Abstract
Influence of infrared low-level laser irradiation (LLLI) on induction of synthesis of some cytokines such as interleykin-1 (Il-1), tumor necrosis factor-α (TNF-α), interferon-γ (INF-γ), interleykin-8 (Il-8) and interleykin-4 (Il-4) by the neutrophils and macrophages in time of bacterial cells phagocytosis that was searched. As the object of analysis we used peritoneal macrophages from white mice and neutrophils from peripheral blood of healthy donors. We used the laser diod with spectrum maximum of 850 nm with doses 300, 900 and 1500 mJ (exposition -60, 180 and 300 s respectively; capacity - 5 mW). We carried out the Enzyme-Linked Immunospot Assay (ELISA) to determine cytokine content during phagocytosis after 3 h and 6 h. We found dynamics in production of the cytokines, which was different for the neutrophils and macrophages. We showed that the infrared LLLI has significant stimulating activity on the proinflammatory cytokines production by neutrophils and macrophages. Moreover we revealed dynamics changing in the Il-8 and Il-4 production.
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Dmitry V. Rudik, Elena I. Tikhomirova, Elena S. Tuchina, "Cytokine production of the neutrophils and macrophages in time of phagocytosis under influence of infrared low-level laser irradiation", Proc. SPIE 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII, 61631S (24 July 2006); doi: 10.1117/12.697429; https://doi.org/10.1117/12.697429
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