Human skin UV-induced fluorescence spectra correction on the internal absorption effect

Irina A. Ovchinnikova; Yuri P. Sinichkin

doi:10.1117/12.695023

7 July 2006 Human skin UV-induced fluorescence spectra correction on the internal absorption effect

Irina A. Ovchinnikova, Yuri P. Sinichkin

Proceedings Volume 6164, Saratov Fall Meeting 2005: Coherent Optics of Ordered and Random Media VI; 61640H (2006) https://doi.org/10.1117/12.695023
Event: Saratov Fall Meeting 2005, 2005, Saratov, Russian Federation

Abstract

The reconstruction method of the human skin intrinsic fluorescence spectra distorted by "the screen effect" was proposed. The method is based on the fact that fluorescence and reflectance spectra are formed by the same absorption and scattering properties of the media. Therefore diffuse reflectance spectrum can be used for fluorescence spectrum reconstruction. The intrinsic fluorescence spectral profiles restoration was tested experimentally using scattering media (phantoms, solutions) with a fluorescent dye and hemoglobin as a strong absorber producing inner-filter distortions. The correction produces the fluorescence spectrum profiles same to the one measured from the corresponding sample with an infinitely low absorption. In this paper we present the results of the fluorescence spectra reconstruction using coefficient &sqrt;R_d(λ) as a correction factor.

Citation Download Citation

Irina A. Ovchinnikova and Yuri P. Sinichkin "Human skin UV-induced fluorescence spectra correction on the internal absorption effect", Proc. SPIE 6164, Saratov Fall Meeting 2005: Coherent Optics of Ordered and Random Media VI, 61640H (7 July 2006); https://doi.org/10.1117/12.695023

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