A novel optical detection system for chromatography applications

W. Meulebroeck; H. Ottevaere; K. Scheir; D. Clicq; G. Desmet; H. Thienpont

doi:10.1117/12.660763

27 April 2006 A novel optical detection system for chromatography applications

W. Meulebroeck, H. Ottevaere, K. Scheir, D. Clicq, G. Desmet, H. Thienpont

Proceedings Volume 6188, Optical Micro- and Nanometrology in Microsystems Technology; 61881I (2006) https://doi.org/10.1117/12.660763
Event: SPIE Photonics Europe, 2006, Strasbourg, France

Abstract

In this work we describe the design of a confocal microscope based optical system which can be used for two types of miniaturized fluid-chromatographic experiments: first of all for the study of shear-driven fluid separations in microchannels and secondly for the study of peak-broadening phenomena in pressure-driven fluids in the same type of microchannels. For the first type of measurements fluorescing dyes (coumarins) are used while for the latter type photo-active molecules (uncaged dyes) like for example fluorescein molecules are needed. We designed the detection system in such a way that by making some small changes to the system we can swap from one application to the other. After calculating the specifications of the optical components, we simulated the system with the sequential ray-tracing-software Solstis and built the system. Finally we did some preliminary experiments which demonstrated the working principle of our setup.

Citation Download Citation

W. Meulebroeck, H. Ottevaere, K. Scheir, D. Clicq, G. Desmet, and H. Thienpont "A novel optical detection system for chromatography applications", Proc. SPIE 6188, Optical Micro- and Nanometrology in Microsystems Technology, 61881I (27 April 2006); https://doi.org/10.1117/12.660763

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available